Technique improvement in establishing mice orthotopic tracheal transplantation surgery model
10.11958/j.issn.0253-9896.2015.10.010
- VernacularTitle:小鼠原位气管移植模型手术技巧及改良
- Author:
Xin WANG
;
Ming DONG
;
Xinglong CHEN
;
Guang LI
- Publication Type:Journal Article
- Keywords:
lung transplantation;
bronchiolitis obliterans;
disease models,animal;
mice,inbred C57BL;
mice,inbred BALB/C
- From:
Tianjin Medical Journal
2015;(10):1122-1124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a simple and effective way of establishing a mice orthotopic tracheal transplantation model in studying bronchiolitis obliterans to reduce operation time. Methods Tracheas from C57BL/6 mice (n=30) were im?planted into Balb/c mice (n=30). Grafts and hosts were matched according to body weight.Orotracheal intubation and endo-stentwas used for trachea anastomose and animal model was establish. Operating time was also noted. Grafts were harvest?ed on Days 15, 30 and 60 after transplantation, using HE staining to observe pathological changes of allograft. Trachea block?ing rate was also calculated. Results Until survival of trachea transplantation, the operative time of donor′s and the recipi?ent′tracheas resection were (7.0±1.0) min and (7.0±1.0) min respectively.Orotracheal intubation the endo-stentestablish?ment cost(1 ± 0.5)min. Trachea anastomose took(20.0 ± 4.0)min and the operation time last(43.0 ± 10.0)min. Conclusion The improvement of orthotopic tracheas transplantation usingOrotracheal intubation the endo-stenfor trachea anastomose is esay-operated and did required complicate skills and instruments with high successful rate. So it is an ideal and stable model in studying bronchiolitis obliterans.
