Effects of Different Extracts ofTangwang Mingmu Granule on Hypoxia-induced Gene Expressions in Vascular Endothelial Cells
10.3969/j.issn.1005-5304.2015.10.014
- VernacularTitle:糖网明目颗粒不同提取部位对缺氧诱导的血管内皮细胞中相关基因表达的影响
- Author:
Changying HAO
;
Mingxia CHEN
;
Wenbin MA
;
Ping GUO
;
Ye LIU
;
Zenglin LIAN
- Publication Type:Journal Article
- Keywords:
Tangwang Mingmu Granule;
diabetic retinopathy;
human umbilical vein endothelial cells;
vascular endothelial cell growth factor (receptor);
ICAM-1;
IL-1α
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(10):45-49
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of different extracts ofTangwang Mingmu Granule on hypoxia induced gene expressions in vascular endothelial cells.Methods COCl2 intervention cells were used to copy hypoxia models. Human umbilical vein endothelial cells EA. Hy926 were divided into blank group, hypoxia model group,Tangwang Mingmu Granule group, extract 1 (glycosides and flavonoids) group, extract 2 (orgain acids and polysaccharides) group and extract 3 (alkaloids) group. The changes in gene expressions of VEGF, VEGFR-1, VEGFR-2, ICAM-1 and IL-1α mRNA were detected by semi-quantitative RT-PCR.ResultsThe gene expression levels of VEGF, VEGFR-1, VEGFR-2, ICAM-1 and IL-1α were significantly up-regulated under the hypoxic condition (P<0.05), and the ratio of VEGFR-1/VEGFR-2 was significantly reduced. However,Tangwang Mingmu Granule significantly reversed the expressions of these genes.Conclusion The function intensity of gene expressions weakens in the following sequence:glycosides and flavonoids>alkaloids>organic acids and polysaccharides inTangwang Mingmu Granule.
