Effects ofZuogui Jiangtang Jieyu Formula on Expressions of Glutamate, NR2A and NR2B in Hippocampus of Diabetic Rats with Depression
10.3969/j.issn.1005-5304.2015.10.020
- VernacularTitle:左归降糖解郁方对糖尿病并发抑郁症大鼠海马谷氨酸及N-甲基-D-天冬氨酸受体2A、2B的影响
- Author:
Yuhong WANG
;
Xiaowen TAN
;
Shang CHAI
;
Hui YANG
;
Rong ZENG
;
Pan MENG
;
Xiuli ZHANG
;
Yuanshan HAN
- Publication Type:Journal Article
- Keywords:
Zuogui Jiangtang Jieyu Formula;
diabetes;
depression;
glutamate;
NR2A;
NR2B;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(10):70-73
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect ofZuogui Jiangtang Jieyu Formula on the expressions of glutamate, NR2A and NR2B in hippocampus of diabetic rats with depression;To explore the mechanism of protective effect. Methods Diabetes with depression rat models were established and then were randomly divided into the model group, positive medicine group, high-, medium- and low-doseZuogui Jiangtang Jieyu Formula groups. Normal rats acted as normal group, 16 rats per group. After 28 days of administration, Open-field test was used to detect the behavior of the rats;glutamate content of hippocampus was detected by ELISA;the expressions of NR2A and NR2B in rat hippocampus were detected by immunofluorescence.Results Compared with normal group, automatic activity times of rats in model group decreased significantly (P<0.01);both glutamate content (P<0.01) and expressions of NR2A, NR2B (P<0.01) increased significantly. Compared with the model group, automatic activity times of rats in positive medicine group and high-doseZuogui Jiangtang Jieyu Formula group significantly increased (P<0.01);glutamate content dropped (P<0.01);expressions of NR2A and NR2B decreased (P<0.05).ConclusionZuogui Jiangtang Jieyu Formula can improve depressive behavior of diabetic rats with depression, which may be related to the regulation of glutamate content and expressions of NR2A and NR2B in hippocampus.
