Effect of MACC1 down-regulation on proliferation of gastric adenocarcinoma cell line
10.11958/j.issn.0253-9896.2015.08.007
- VernacularTitle:MACC1基因下调对胃腺癌细胞的生长抑制作用
- Author:
Yi LUO
;
Lupei LIU
;
Lu YU
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
adenocarcinoma;
cell proliferation;
cell cycle;
RNA interference;
MACC1;
MGC-803 cell
- From:
Tianjin Medical Journal
2015;(8):856-859
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of MACC1 down-regulation on the growth of gastric adenocarcinoma cells. Methods siRNA (MACC1-siRNA1 and MACC1-siRNA2) that can transiently silenced MACC1 was designed, syn?thesized and transfected into MGC-803 cells by lipofectamine 2000. Non-specific siRNA was transfected to be used as nega?tive control. The efficiency of MACC1 depletion was determined by Real-time quantitative PCR. MTT, colony formation and flow cytometry assay were performed to examine cell proliferation. The expressions of MACC1, P21,CDK4, CCND1 and c-myc were determined by Western blot. Results Compared with cells in negative control group, transiently silencing MACC1 decreased the expression of MACC1 in MGC-803 cells shown by Real-time PCR. MACC1 downregulation drastical?ly changed the proliferation, colony formation and cell cycle of gastric adenocarcinoma cells in vitro ( P<0.05). The expres?sions of MACC1 , CDK4, CCND1 and c-myc proteins in cells of MACC1 silence group were much lower while P21 expres?sion level was much higher than those in negative control. Conclusion Down-regulation of MACC1 result in blocking cell cycle, inhibiting proliferation of MGC-803 cells. So it may serve as a promising target in the treatment of gastric cancer.
