The correlation study between the manifestation of MRI and pathology of early cerebral infarction after treatment with AQP4 mRNA silence
10.3969/j.issn.1002-1671.2015.09.035
- VernacularTitle:AQP4基因沉默治疗早期脑梗死的磁共振成像与病理研究
- Author:
Zhanping HE
;
Hong LU
- Publication Type:Journal Article
- Keywords:
ischemic;
magnetic resonance imaging;
pathology;
Aquaporin-4;
gene silencing
- From:
Journal of Practical Radiology
2015;(9):1539-1543
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study investigated magnetic resonance imaging (MRI)manifestations and pathological changes after aquaporin-4 (AQP4)gene silencing in treatment of early cerebral infarction.Methods Ninety adult Wistar rats were randomly divided into con-trol group,infarction group and treatment group.All the above groups were assigned to five sub-groups according to the time point:0.5,1,2,4 and 6 hours,respectively (n= 6 for each group).For the right middle cerebral artery occlusion (MCAO-infarction group),the rats were separately injected with short hairpin RNA-AQP4 liposome (shRNA-AQP4+MCAO-control group)and in-terference agents (siRNA-AQP4 + MCAO-interference group).The rats in all groups were examined with T2 weighted image (T2 WI)and diffusion-weighted imaging (DWI).The rs-T2 WI,rs-DWI and relative apparent diffusion coefficient (ADC)of the big-gest high-signal-intensity layer on T2 WI and DWI were measured.Corresponding brain tissue was collected for pathological observa-tion.The data were analyzed using SPSS 13.0 software.Results Identical changes were detected between control and infarction groups.The high-signal intensity was found on DWI at 0.5 hour and on T2 WI at 2 hours after MCAO.rs-DWI and rs-T2 WI in-creased with time.The value of relative ADC decreased quickly within 2 hours after MCAO,and cytotoxic edema was significantly aggravated.The value of relative ADC increased slowly at 4 and 6 hours,but angioedema was detected during this period.There was significant change in relative ADC between the treatment and control groups at 0.5 to 4 hours (P < 0.01)and cytotoxic edema was significantly lightened.But there was no significant difference in relative ADC between treatment and control groups at 6 hours (P >0.05).However,angioedema was visible in this group at 6 hours.Above results confirmed that during early cerebral in-farction,the differential regions of DWI and T2 WI high-signal area were considered as cytotoxic edema,which was the pathologic basis of the decreased ADC values.AQP4 gene silencing could effectively reduce cytotoxic edema.Conclusion DWI and ADC value could reveal timely and exactly therapeutic outcomes of cytotoxic edema.The high-signal intensity on T2 WI represents angioedema, and AQP4 gene silencing has no obvious change.
