Serosurveillance and establishment of a reverse transcription-polymerase chain reaction assay for bovine parainfluenza virus type 5.
10.14405/kjvr.2015.55.3.185
- Author:
Dong Kun YANG
1
;
Sung Suk CHOI
;
Beom Joo LEE
;
Ha Hyun KIM
;
Hyun Ye JO
Author Information
1. Viral disease division, Animal and Plant Quarantine Agency, Anyang 430-757, Korea. yangdk@korea.kr
- Publication Type:Original Article
- Keywords:
bovine;
parainfluenza virus 5;
reverse transcription-polymerase chain reaction;
serosurveillance
- MeSH:
Animals;
Cattle;
Gyeonggi-do;
Gyeongsangbuk-do;
Gyeongsangnam-do;
Jeollabuk-do;
Parainfluenza Virus 5;
Paramyxoviridae Infections*;
Sensitivity and Specificity
- From:Korean Journal of Veterinary Research
2015;55(3):185-189
- CountryRepublic of Korea
- Language:English
-
Abstract:
Bovine parainfluenza virus type 5 (bPIV5) was isolated from cattle with downer cow syndrome in 2012, and included both respiratory and neurotropic pathogens from a variety of animals. In the current study, we conducted serosurveillance using sera obtained from seven Korean farms and optimized a reverse transcription-polymerase chain reaction (RT-PCR) assay to detect bPIV5. The overall seropositive rate for Korean cattle was 21.4% (163/760). A farm located near the city of Milyang in Gyeoungnam province had a markedly elevated seropositive rate for bPIV5 compared to that of the other six farms. The regional seropositive rates were 4.2% (8/192) for Haman, 19.5% (18/55) for Hwasung, 73.9% (65/88) for Milyang, 26.0% (50/192) for Namwon, 1.0% (1/96) for Uljin, 13.5% (13/96) for Yeongju, and 32.7% (8/41) for Yongin. The sensitivity and specificity of three RT-PCR primer sets used to amplify the conserved fusion gene of bPIV5 were also evaluated. An RT-PCR assay using the bPIVFR3 primer set was 10-fold more sensitive than the assays using the two other primer sets and did not result in non-specific amplification. These results demonstrated that the bPIFR3 primer set can be used to detect bPIV5.
