RNase MC2 manifests antitumor effects towards human hepatocellular carcinoma

VernacularTitle:苦瓜子有效成分RNA酶MC2抑制肝癌细胞生长
Author: Shuguang SU ; Xiaohua WANG ; Zhen ZHAO ; Jingfeng GUO
Publication Type:Journal Article
Keywords: liver neoplasms; RNase MC2; cell cycle; autophagy
From: Chinese Journal of Clinical and Experimental Pathology 2015;(8):890-894
CountryChina
Language:Chinese
Abstract: Purpose To determine the effect of RNase MC2 purified from momordica charantia on cell growth of hepatocellular carcino-ma ( HCC) and its underlying mechanism. Methods MTT, colony formation and nude mice model were used to examine the activity of RNase MC2 in cell proliferation. Cell cycle analysis was done by flow cytometry. Autophagy induced by RNase MC2 treatment was observed via transmission electron microscope. Western blot was performed to detect the RNase MC2-mediated changes of proteins. Re-sults In vitro and in vivo data showed that RNase MC2 markedly inhibited HCC cell proliferation, arrested cells at G2/M phase by in-creasing expression of p53 and p21, induced autophagy via upregulating Beclin-1 and LC3-Ⅱ. Furthermore, combination of RNase MC2 and Sorafenib exerted enhanced lethal effect on HCC cells. Conclusion RNase MC2 manifests significant antitumor activities and enhances the killing effect of Sorafenib in HCC via inducing cell cycle arrest and autophagy.