Anti-inflammatory effects of 4,4'-diaminodiphenyl sulfone (dapsone) in lipopolysaccharide-treated spleen cells: selective inhibition of inflammation-related cytokines.
10.14405/kjvr.2015.55.3.199
- Author:
Sun Young MOON
1
;
Hong Gu JOO
Author Information
1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. jooh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
anti-inflammatory activity;
dapsone;
inflammatory cytokines;
spleen cells
- MeSH:
Animals;
Apoptosis;
Bacteria;
Cytokines*;
Dapsone;
Dinoprostone;
Inflammation;
Interleukin-6;
Interleukins;
Leprosy;
Mice;
Mycobacterium leprae;
Neutrophils;
Nitric Oxide;
Spleen*;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Veterinary Research
2015;55(3):199-204
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
4,4'-diaminodiphenyl sulfone (dapsone) is a sulfone drug that has antibacterial effects on a variety of bacteria, especially Mycobacterium leprae; thus, it has been used to treat leprosy. Previous studies demonstrated that dapsone inhibits integrin-mediated adherence of neutrophils and production of prostaglandin E2 by polymorphonuclear leukocytes. Hence, dapsone may act in immune cells and regulate cell-mediated inflammation processes. However, its anti-inflammatory effects remain unclear. The present study demonstrated that dapsone modulates the production of inflammation-related cytokines in immune cells. We employed the spleen cells of mice, which are major immune cells, and lipopolysaccharide (LPS) as a causative agent of inflammation for experiments. Dapsone induced a proportional change in splenocyte subsets and the apoptosis of spleen cells. Interestingly, dapsone decreased the production of tumor necrosis factor-alpha and interleukin (IL)-10, but not IL-6, in LPS-treated spleen cells. In other assays, we measured the dapsone-induced production of nitric oxide (NO) and the expression of activation markers of spleen cells. Dapsone decreased NO production in LPS-treated spleen cells. Taken together, our results demonstrate that dapsone has anti-inflammatory effects in immune cells and provide new insight into the potential uses of this agent.
