Application of cell block combined with immunohistochemistry in diagnosis of pleural effusion
10.13315/j.cnki.cjcep.2015.08.015
- VernacularTitle:细胞块联合免疫组化在胸腔积液诊断中的应用
- Author:
Lihua LUO
;
Wanyi ZHANG
;
Huijuan LIU
;
Bingquan LI
;
Shigang WU
;
Jinghua XIAO
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma cell;
pleural effusion;
immunohistochemistry;
cell block
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(8):904-907
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the application value in diagnosis of pleural effusion by cell block combined with immunohisto-chemistry. Methods 60 cases of pleural effusion were collected, and paraffin-embedded cell block was prepared and immunohisto-chemistry was used to detect the expression of CK7, TTF-1, E-cadherin, CEA and Calretinin. Results By use of cell block combined with immunohistochemistry, malignant detected rate was higher than that of the conventional centrifugal smear. There was statistical significance in the expression of CK7, TTF-1, E-cadherin, CEA and Calretinin between pleural effusion lung adenocarcinoma and reac-tive hyperplasia mesothelial cells (P<0. 05). CK7, TTF-1, E-cadherin and CEA were highly expressed in pleural effusion of lung ad-enocarcinoma cell. Calretinin was highly expressed in hyperplastic mesothelial cells. Conclusion Cell block and immunohistochemi-cal technique combination in the differential diagnosis of difficult pleural effusion has important clinical significance. It is worthy of popularization and further clinical application.
