The effects of 2-hydroxyethyl methacrylate on the proliferation and migration of human dental pulp cells
10.3969/j.issn.1001-3733.2015.05.011
- VernacularTitle:甲基丙烯酸β-羟乙酯对人牙髓细胞增殖和迁移的影响
- Author:
Su SUN
;
Ming FANG
;
Lijuan SHEN
;
Guanlin WANG
;
Hailang DIWU
;
Yuhong XIAO
- Publication Type:Journal Article
- Keywords:
HEMA;
Dental pulp cells;
Proliferation;
Migration
- From:
Journal of Practical Stomatology
2015;(5):648-652
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the effects of HEMA on the proliferation and migration of human dental pulp cells(hDPCs).Meth-ods:hDPCs were obtained using tissue explant culture technique in vitro,and then cells of the 3rd -5th passages were treated by differ-ent concentrations of HEMA for 24,48 and 72 h respectively.Cell proliferation was examined by MTT assay.Cell migration was ob-served by Transwell method.Results:The proliferation ability of hDPCs decreased when exposed to HEMA in both time and concentra-tion dependent manner(vs control,P <0.05).Cell proliferation at 24 h expossure was statistically higher than that at 48 h and 72 h(P<0.05).The migration of hDPCs was significantly reduced in HEMA groups at different concentrations(vs control,P <0.05).Con-clusion:HEMA inhibits the proliferation and migration of human dental pulp cells in vitro.
