Simultaneous Content Determination of Gensinoside Rg1, Re and Rb1 in Panacis Japonici Rhizoma by HPLC
10.3969/j.issn.1005-5304.2015.10.021
- VernacularTitle:HPLC测定竹节参中人参皂苷Rg1、Re、Rb1含量
- Author:
Hong ZHENG
;
Quan GU
;
Lei WANG
;
Haiyan ZOU
- Publication Type:Journal Article
- Keywords:
Panacis Japonici Rhizoma;
ginsenoside;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(10):74-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for simultaneous content determination of ginsenoside Rg1, Re and Rb1 in Panacis Japonici Rhizoma.Methods Chromatographic separation was carried out by using an Agilent Poroshell 120 C18 column (4.6 mm × 100 mm, 2.7μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution at a flowrate of 1.0 mL/min, and the injection volume was 10μL. The detection wavelength and column temperature were 203 nm and 30℃ respectively.Results Ginsenoside Rg1, Re and Rb1 had the baseline separation and were in good linear range. The recovery rates were 99.5%, 103.0% and 100.5% respectively.Conclusion The approach is simple, accurate, with good repeatability and short analysis period, which can determine the contents of ginsenoside Rg1, Re and Rb1 correctly and provide references for quality control of Panacis Japonici Rhizoma.
