Content Determination of Ginsenoside Rg1, Ginsenoside Rb1 and Notoginsenoside R1 in Tianqi Tongjing Capsule by HPLC
10.3969/j.issn.1005-5304.2015.10.022
- VernacularTitle:HPLC测定田七痛经胶囊中人参皂苷Rg1、Rb1及三七皂苷R1含量
- Author:
Liang CHEN
;
Yifan QIAN
- Publication Type:Journal Article
- Keywords:
Tianqi Tongjing Capsule;
HPLC;
Ginsengnoside Rg1;
Ginsengnoside Rb1;
Notoginsenoside R1
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(10):77-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for content determination of Ginsengnoside Rg1, Ginsengnoside Rb1 and Notoginsenoside R1 inTianqi Tongjing Capsule.Methods An Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5μm) was used. The mobile phase was composed of acetonitrile (A) and water (B) with gradient elution (0-20 min, 19%→21%A;20-50min, 21%→36%A) at a flow rate of 1.0 mg/mL;the wavelength of detector was 203 nm;the temperature of the column was 25℃.Results The calibration curves of Ginsengnoside Rg1, Ginsengnoside Rb1 and Notoginsenoside R1 showed good linearity within the range of 0.442 4-3.981 6μg (r2=1.000 0), 0.524 8-3.673 6μg (r2=0.999 4) and 0.203 2-1.016μg (r2=0.999 2), respectively. The average recoveries (n=9) were 99.49%, 99.02% and 99.98%, and RSD were 2.44%, 2.45% and 2.14%, respectively.Conclusion The method is simple, reliable, rapid and with good repeatability, and can effectively control the quality ofTianqi Tongjing Capsule.
