Procyanidin inhibits multidruy resistance 1 mRNA expression via regulating microRNA-27a and microRNA-451

VernacularTitle:原花青素通过调节微小RNA-27a与微小RNA-451抑制多药耐药基因1 mRNA的表达
Author: Qing ZHANG ; Boxin ZHAO ; Shengqi WANG ; Qianying LIANG ; Fuheng YANG ; Yatian WANG ; Yun CAI ; Guofeng LI
Publication Type:Journal Article
Keywords: procyanidin; microRNA; multidrug resistance1; stem-loop PCR
From: Journal of International Pharmaceutical Research 2015;(4):501-506
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of expression of microRNA-27a(miR-27a) and microRNA-451(miR-451) in A2780/T cells and its relativity to multidrug resistance (MDR)1 mRNA inhibition by procyanidin. Methods Stem-loop PCR method was performed to evaluate the expression of miR-27a and miR-451 in use of procyanidin (0-40μmol/L) in 0-48 h in A2780/T cells. Additionally, over-expressing or interfecting microRNAs by using mimics or inhibitor of miR-27a and miR-451, the expression of MDR1 mRNA was assessed by RT-PCR in cells exposing to procyanidin. Results The expression of miR-27a and miR-451 was significant inhibited by procyanidin in both time- and concentration-dependency. Over-expressed MDR1 mRNA associated with miR-27a or miR-451 mimics was blocked by procyanidin, whereas there was no effect on down-expressed MDR1 mRNA associated with miR-27a or miR-451 inhibitor by procyanidin. Conclusion Procyanidin inhibits MDR1 mRNA expression by inhibiting miR-27a and miR-451 expression in A2780/T cells.