Analysis of protein tyrosine phosphatase 1B gene mutation in myeloproliferative neoplasms

VernacularTitle:骨髓增殖性肿瘤患者蛋白酪氨酸磷酸酶1B基因突变分析
Author: Lili ZHOU ; Jianfei FU ; Hao WU ; Bing LI ; Aibin LIANG
Publication Type:Journal Article
Keywords: Myeloproliferative neoplasms; Protein tyrosine phosphatase 1B; DNA sequencing
From:Journal of Leukemia & Lymphoma 2015;24(7):424-427
CountryChina
Language:Chinese
Abstract: Objective To analyse protein tyrosine phosphatase 1B (PTP1B) gene mutation in myeloproliferative neoplasms (MPN).Methods DNA sequencing technology was used to detect DNA sequences of PTP1B in MPN patients (n =84) and normal controls (n =37).Results For Exon1-6,Exon9 and Exon10,84 cases of MPN patients and 37 cases of control group were not detected mutation.For EXON 8,18 of 84 MPN patients had Exon8 C/T heterozygous mutation and 10 of 37 normal controls were detected C/T heterozygous mutation.There was no significant difference between MPN patients and normal controls (x2 =0.453,P =0.501).Exon7 was detected in 38 MPN patients and 2 cases of patients were found C/T heterozygous mutation,while in the control group,1 case with G/C heterozygous mutation.All of the cases were not detected homozygous mutation.Conclusion Using DNA sequencing technology to detect gene mutations of PTP1B,there is no significant difference between MPN patients and normal controls.