Effects of interleukin-1βon MMP-13 expression in rat chondrocytes and its regulation of miR-27b
10.11958/j.issn.0253-9896.2015.08.011
- VernacularTitle:白细胞介素-1β对大鼠软骨细胞MMP-13表达的影响及miR-27b的调控作用
- Author:
Xin WANG
;
Yu QIN
- Publication Type:Journal Article
- Keywords:
chondrocytes;
matrix metalloproteinase 13;
microRNAs;
interleukin-1beta;
microarray analysis;
miR-27b
- From:
Tianjin Medical Journal
2015;(8):871-875
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of interleukin-1β(IL-1β) on expression of Matrix Metalloproteinases 13 (MMP-13) in rat chondrocytes and its regulation of miR-27b. Methods Chondrocytes were extracted from 7 Wistar male rats. Expression of MMP-13 were examined by Western blot at 0 h, 24 h, 48 h after IL-1βstimulation. Differential miRNAs expression profiles were examined by miRNAs microarray. The most obviously down-regulated miRNAs were confirmed by quantitative Real-time PCR. Targeted regulation relationship between miR-27b and MMP-13 was set up by Luciferase re?porter gene experiments. Results Expression of MMP-13 in rat chondrocytes was increased at a timely dependent manner upon IL-1βstimulation(P<0.05);Microarray revealed 36 miRNAs whose expression changed, among which 6(miR-27b, miR-31, miR-26a, miR-26b, miR-23, miR-204)were especially obvious. Real-time PCR confirmed that miR-27b was the one whose expression level were most down-regulated. Transient co-transfection of miR-27b mimics with luciferase expres?sion plasmids resulted in significant repression of luciferase activity in rat chondrocytes (P < 0.05). Conclusion IL-1βstimulation result in down-regulation of miR-27b and up-regulation of MMP-13 expression. MiR-27b and MMP-13 show targeted regulation relationship.
