Study of regulation of miR-21 on ADAMTS-1 expression
10.3969/j.issn.1006-5725.2015.15.005
- VernacularTitle:miR-21对ADAMTS-1的调节在肺纤维化体内外实验中的研究
- Author:
Hong ZHAO
;
Pingbo YAO
;
Li DAI
;
Ping ZHONG
;
Jun HE
- Publication Type:Journal Article
- Keywords:
miR-21;
In vivo and in vitro experiment;
ADAMTS-1;
Pulmonary Fibrosis
- From:
The Journal of Practical Medicine
2015;31(15):2426-2429
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effect of miR-21 on ADAMTS-1 expression in pulmonary fibrosis model. Methods A mouse model of pulmonary fibrosis was established using bleomycin (BLM) anda model of pulmonary fibrosis was constructed in vitro , the expression level of miR-21 was measured by quantitative real-time polymerase chain reaction (qRT-PCR), while the protein expression of ADAMTS-1 was measured by Western blot. NIH3T3 were transfected with miR-21 mimics and inhibitor in vitro and the cellular expression of ADAMTS-1 was measured by Western blot. Results Compared with the blank group , in mouse models of pulmonary fibrosis , the miR-21 expressions in lung tissues at three time points after BLM-treatment were significantly up-regulated while an evident decrease in ADAMTS-1 expressions were observed (P < 0.01). In vitro pulmonary fibrosis model , NIH3T3 cells after TGF-β1 in concentration 5 μg/L stimulation down-regulated ADAMTS-1 expression and up-regulated miR-21 expression (P < 0.01). NIH3T3 transfected with miR-21 mimics and inhibitor, up-regulated miR-21 expression, while down-regulated ADAMTS-1 protein expression. Conclusions Up-regulation of miR-21 and Down-regulation of ADAMTS-1 might be involved in the progression of pulmonary fibrosis model; miR-21 could negatively regulate ADAMTS-1 expression.
