The effects of electrospun nanofibers loading icariin on osteoblast MC3T3-E1 cells

VernacularTitle:载淫羊藿苷缓释电纺纤维对成骨细胞影响的实验研究
Author: Tongbin LIU ; Yue YUAN ; Yuting ZHAO ; Jingjing WANG ; Weiyan MENG
Publication Type:Journal Article
Keywords: Icariin(ICA); Controlled release; Osteoblast; Poly-L-lactic acid(PLLA)
From: Journal of Practical Stomatology 2015;(6):770-775
CountryChina
Language:Chinese
Abstract: Objective:To prepare poly-L-lactic acid(PLLA)electrospun nanofibers carrying icariin(ICA)(ICA /PLLA)and to evaluate the effects of the ICA /PLLA on MC3T3-E1 cells.Methods:ICA solution was dispersed into PLLA solution,and electrospun fibers were fabricated by W/O emulsion method.The morphology of ICA /PLLA was observed by SEM.The in vitro release kinetics of ICA /PLLA was examined.The attachment of MC3T3-E1 cells on ICA /PLLA was examined by propidiumiodide(PI)labling and ob-served under fluorescent microscope.The proliferation of the cells was measured by MTT assay.The differentiation of the cells was ob-served by alkaline phosphatase (ALP)assay.Results:In vitro,ICA was effectively released from ICA /PLLA for 22 days,cells were attached well on the surface in all groups,ICA did not affect the proliferation of MC3T3-E1 cells(P >0.05),but increased the ALP activity(P <0.05)of the cells.Conclusion:ICA /PLLA can effectively control the release of ICA and promote the differentiation of MC3T3-E1 cells.