Ameliorative effects of type-A procyanidins polyphenols from cinnamon bark in compound 48/80-induced mast cell degranulation.
10.5115/acb.2017.50.4.275
- Author:
Amit D KANDHARE
1
;
Urmila M ASWAR
;
Vishwaraman MOHAN
;
Prasad A THAKURDESAI
Author Information
1. Department of Scientific Affairs, Indus Biotech Private Limited, Pune, India. prasad@indusbiotech.com
- Publication Type:Original Article
- Keywords:
Cinnamomum zeylanicum;
Type-A procyanidin polyphenols;
Mast cell degranulation inhibition
- MeSH:
Animals;
Asthma;
Cinnamomum zeylanicum*;
Developing Countries;
Histamine;
Interleukin-4;
Mast Cells*;
Polyphenols*;
Proanthocyanidins*;
Rats;
Rhinitis, Allergic
- From:Anatomy & Cell Biology
2017;50(4):275-283
- CountryRepublic of Korea
- Language:English
-
Abstract:
Allergic diseases are a significant health concern in developing countries. Type-A procyanidin polyphenols from cinnamon (Cinnamomum zeylanicum Blume) bark (TAPP-CZ) possesses antiasthmatic and antiallergic potential. The present study was aimed at the possible anti-allergic mechanism of TAPP-CZ against the compound 48/80 (C48/80)–induced mast cell degranulation in isolated rat peritoneal mast cells (RPMCs). TAPP-CZ (1, 3, 10, and 30 µg/ml) was incubated for 3 hours with isolated, purified RPMCs. The C48/80 (1 µg/ml) was used to induce mast cell degranulation. The mast cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay whereas histamine, β-hexosaminidase (β-HEX), and interleukin-4 (IL-4) levels were determined in RPMCs. TAPP-CZ (3, 10, and 30 µg/ml) showed significant and dose-dependent decrease in a number of degranulated cells and levels of markers (histamine, β-HEX, and IL-4) as compared with C48/80 control. In conclusion, TAPP-CZ stabilizes mast cell and cause inhibition of the allergic markers such as histamine, IL-4, and β-HEX in IgE-mediated manner. The present study supports mast cell stabilization as a possible mechanism of action of TAPP-CZ against immune respiratory disorders such as asthma and allergic rhinitis.
