Construction of rat models of acute high altitude stress-induced gastrointestinal injury
10.3969/j.issn.2095-4344.2015.40.015
- VernacularTitle:构建急进高海拔地区急性应激性胃肠道损伤大鼠模型
- Author:
Zhihua YANG
;
Kun WANG
;
Xiaoqin HA
;
Ping ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(40):6475-6479
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:At present, there are few reports about construction of the model of acute high altitude stress-induced gastrointestinal injury. Construction of a stable high altitude stress-induced gastrointestinal injury model has become a premise of the current studies on acute stress-induced gastrointestinal injury and related diseases. OBJECTIVE: To establish rat models of acute high altitude stress-induced gastrointestinal ischemia/reperfusion injury. METHODS:Twenty-four male Wistar rats were randomly divided into normal control (lower limit of high altitude) , sham (high altitude) and ischemia/reperfusion (high altitude) groups (n=8 rats/group). Rats from al groups underwent a rush for high altitude region. The root of the superior mesenteric artery of rats in the ischemia/reperfusion group was clamped by a vascular clip to completely block blood flow for 60 minutes, and then intestinal blood flow was recovered for reperfusion for 60 minutes. The rats in the sham group were only
