Efefct of adoptive transfer of 5 AzaD-induced re gulator T cells on outcomes of gestation in abortion-prone murine matings
10.3969/j.issn.1000-484X.2015.10.003
- VernacularTitle:5－氮－2′－脱氧胞苷诱导的调节性T细胞转输对自然流产孕鼠妊娠结局的影响
- Author:
Jian WANG
;
Fuxi ZHAO
;
Jing YANG
;
Runhua LIU
;
Yaqin MU
;
Xiying WANG
;
Juncheng GUO
;
Lihua LIU
;
Guangmei BAI
- Publication Type:Journal Article
- Keywords:
5-aza-2′-deoxycytidine;
CD4+CD25+Foxp3+Treg;
Adoptivetherapy;
Recurrentspontaneousabortion
- From:
Chinese Journal of Immunology
2015;(10):1310-1314
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of the adoptive transfer of CD4+CD25+Foxp3+regulatory T cells ( iTregs) induced by 5-aza-2′-deoxycytidine (5AzaD) on pregnant outcome of the abortion-prone mice.Methods:Sixty cases of female CBA/J × male DBA/2J abortion-prone matings were taken as study group,the CD4+T cells from spleen of twenty female CBA/J mice were separated by magnetic activated cell sorting (MACS),5AzaD was applied to the conversion of CD4+CD25-T cells to iTregs,the expression of Foxp3 in Tregs was characterized by flow cytometry analysis before and after epigenetic modification.The purified iTregs were injected into abortion-prone mice on day 1 or 4 of pregnancy,respectively,which were used as therapy groups,and then the embryo resorption rate was counted on day 14 of pregnancy.Results:After the treatment of 5AzaD,the percentage of iTregs in CD4+T cells was (41.50±8.03)%.The embryonic absorption rates of the two therapy groups were 10.47%(on day 1 of pregnancy) and 21.69%(on day 4 of pregnancy) ,respectively ( P<0.05 ) .Conclusion: Epigenetic modication of CD4+CD25-T cells may solve the problem of nTregs deficiency,particularly adoptive therapy of 5AzaD-induced iTregs at early stage of pregnancy can maintain normal pregnancy.
