Immunomodulatory effects of butyrate on bone marrow derived dendritic cells
10.3969/j.issn.1000-484X.2015.10.004
- VernacularTitle:丁酸对小鼠骨髓源树突状细胞的免疫调节作用
- Author:
Yetao QIANG
;
Shuiyun WU
;
Mutian HAN
;
Lu CHENG
;
Huimin JIN
;
Cheng YAN
;
Chong LI
;
Xia LIU
;
Miaomiao ZHANG
;
Qixiang SHAO
;
Sheng XIA
- Publication Type:Journal Article
- Keywords:
Butyrate;
Dendritic cell;
Costimulatory molecule;
Cytokine;
CD8+T;
TLR4
- From:
Chinese Journal of Immunology
2015;(10):1315-1319
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of butyrate produced by bacterial metabolism on immune features of murine bone marrow-derived dendritic cells(BMDCs) and its potential mechanisms.Methods: BMDCs were prepared from bone marrow cells of C57BL/6 mice by being cultured with GM-CSF and IL-4.The expression of CD80,CD86,B7-DC and MHCⅡ on BMDCs and its pri-ming ability on the proliferation of OVA257-264 antigen specific CD8+T cell were analyzed by using Flow cytometry.The mRNA levels of IL-6 and IL-12 in BMDCs were detected by real-time fluorescence quantitative PCR(q-PCR).Simultaneously,Griess reaction and Western blot was used for analyzing the levels of NO2-in BMDCs culture supernatant and the ERK phosphortylation in BMDCs respectivly.Results:Butyrate could decrease the levels of CD80,CD86,MHCⅡand B7-DC,and downregulate the capability of BMDCs in priming the proliferation of CD8+T cells.Furthermore,the secretions of IL-6,IL-12,NO2-and the phosphorylation of ERK were sup-pressed.Conclusion:Butyrate down-regulats the immune functions of BMDCs via inhibition of ERK phosphorylation in TLR 4 signaling pathway.
