Screening and identification of peptide mimics to lipoteichoic acid by phage displayed random peptide library
10.3969/j.issn.1000-484X.2015.10.015
- VernacularTitle:模拟金黄色葡萄球菌脂磷壁酸表位的初步筛选与鉴定
- Author:
Xiangyu WANG
;
Zhaoxia HUANG
;
Xiaorui HOU
;
Ping ZHU
;
Ning FU
;
Beiyi LIU
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
Phage display peptide library;
Lipoteichoic acid;
Mimic peptide
- From:
Chinese Journal of Immunology
2015;(10):1366-1369
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen epitope mimics to lipoteichoic acid from a random 12-mer phage display peptide library and i-dentify the specificity of the mimotopes of LTA.Methods:The monoclonal antibody against LTA was used as a target to screen the 12-mer phage display peptide library and the specificity of phage clones were identified by sandwich ELISA.The amino acid sequences of positive phage clones were deduced from DNA sequencing.The specificity of synthetic peptide were identified by sandwich ELISA.Results:4 clones were obtained after 3 rounds of screening.Amino acid sequence analysis revealed four different types of mimotope sequence.A linear peptide (GHxDFRQxxQPS),named L2,which derived from positive sequence was synthesized.ELISA result indicates that L2 can bind to anti-LTA mAb specifically in a dose-dependent manner.Conclusion:The mimotopes of LTA were obtained by using the phage display technology.
