Intravenous injection of bleomycin induces pulmonary fibrosis in mice：a stability evaluation
10.3969/j.issn.2095-4344.2015.40.008
- VernacularTitle:静脉注射博来霉素诱导肺纤维化模型小鼠的稳定性评价
- Author:
Changli TU
;
Xiang LIU
;
Xiaobin ZHENG
;
Jialin YU
;
Shuqin ZHU
;
Minhong SU
;
Weiming WU
;
Jin HUANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(40):6436-6443
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:It is particularly important to establish an ideal animal model of pulmonary fibrosis to investigate the underlying pathogenesis and screen effective drugs to prevent and control pulmonary fibrosis. OBJECTIVE: To establish a modified scheme of establishing mouse models that can reflect pulmonary fibrosis formation in humans. METHODS: Fifty-six male C57BL/6 mice were randomly divided into two groups: A (a single large-dose injection) and B (multiple smal-dose injections). Mice in group A were subjected to a single intravenous injection of bleomycin 200 mg/kgviathe tail vein; and mice in group B received intravenous injections of bleomycin 50 mg/kg via the tail vein per week, totaly for 6 weeks.
