Separation and purification of total alkaloids from Evodia rutaecarpa using 732 cation exchange resin and investigations on its anti-breast cancer bioactivity
- VernacularTitle:732型阳离子树脂分离纯化吴茱萸总碱及抗乳腺癌研究
- Author:
Shixue CHEN
;
Yuanyong YAO
;
Zhongying LU
;
Bangcheng TANG
- Publication Type:Journal Article
- Keywords:
Evodia rutaecarpa;
cation exchange resin;
extraction process;
total alkaloids
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(9):15-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize conditions for extraction of alkaloids from Evodia rutaecarpa by means of 732 cation exchange resin and performe its anti-breast cancer bioactivity.Methods The optimum processing route on extraction of alkaloids from Evodia rutaecarpa was investigated by means of 732 cation exchange resin.The performance of 732 cation exchange resin was compared with tranditonal process ( aqueous extraction-ethanol precipitation in extraction-purification process).The alkaloid reagent-potassium mercuric iodide test and MTT test were performed on the crudes.Results Compared with traditional purification process, it was much better to use 732 cation exchange resin approach for extraction of alkaloids from Evodia rutaecarp with high yield(16.81%) The best eluent should be saturated brine with excellent purification.No obvious correlations were found between the toxin of breast cancer cell and concentration of total alkaloids.When the concentration of total alkaloids was 50μmol/mL, cellular survival rate was 68%afterward 24 h.When the concentration of total alkaloids comes to 100 μmol/mL and 150 μmol/mL, cellular survival rates were slightly decreased by 66% and 60%.Conclusion 732 cation exchange resin performes much higher than traditional process in purification of total alkaloids extracted from Evodia rutaecarpa.Simultaneously, the extracted total alkaloids showe remarkable inhibition in breast cancer cell.
