Denervation effects on bone regeneration and expression of Runx2 during distraction osteogenesis
10.3969/j.issn.2095-4344.2015.37.016
- VernacularTitle:失神经支配在胫骨牵张成骨延长过程中的骨再生及Runx2表达
- Author:
Ke ZHENG
;
Donghui SONG
;
Xingmei FENG
;
Songsong ZHU
;
Jing HU
;
Bin YE
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(37):5988-5992
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:During the healing of fractures, removal of sciatic nerve can result in insufficient mechanical rigidity of newborn woven bone. However, there are less reports concerning the denervation effects during distraction osteogenesis. OBJECTIVE:To observe the effect of removal of the sciatic nerve on bone regeneration and the expression of Runt-related transcription factor 2 (Runx2) protein during distraction osteogenesis in a rabbit model. METHODS:Twenty-four adult male New Zealand rabbits were selected and underwent left tibial osteodistraction to construct animal models of distraction osteogenesis. Before distraction, the animals were randomly divided into group R (resecting the left sciatic nerve) and group I (intact left sciatic nerve). Six weeks after completion of distraction, the animals were kil ed and the lengthened tibias were harvested for radiography, three-dimensional CT reconstruction, histological evaluation, connectivity density (Conn.D) evaluation. RESULTS AND CONCLUSION:New regenerated bone was present and Runx2 protein was expressed in the distraction gaps of al animals at the end of the study, as revealed by radiography, three-dimensional CT reconstruction, and histological observation. However, less new bone formation and a lower degree of mineralization and expression of Runx2 protein were observed in group R compared with group I. The results suggest that the denervation appears to have an inhibitory effect on bone formation and the expression of Runx2 protein during distraction osteogenesis.
