Comparison of two protein extraction methods from velvetantler
- VernacularTitle:鹿茸蛋白2种不同提取方法的比较研究
- Author:
Chaohua LI
;
Yi WANG
;
Zhongmei HE
;
Yanan SUN
- Publication Type:Journal Article
- Keywords:
velvet antler;
protein extraction;
SH-SY5Y
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(9):23-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare optimal protein extraction method of velvet antler ( VA ) and explore the repairmen activity of velvet antler proteins ( VAPs ) on 1-methyl-4-phenylpyridinium ( MPP +) damaged nerve cell.Methods VAPs was obtained from fresh velvet antler by homogenization and micronization method respectively.The protein content of VAPs was measured by Bradford and the molecular weight was identified by SDS-PAGE.Scanning electron microscope (SEM) was perfomed to observe the ultrastructure of VAPs.Neuroblastoma cell line (SH-SY5Y) damage was induced by MPP +, and activity of each compound was compared by the proliferation of damaged cell detected by MTT method.Results The yield of micronization method of VAPs was higher than homogenization method and with better characters both in SDS-PAGE and SEM.Activity detection indicated that VAPs at the concentration of 125 μg/mL by homogenate method could significantly increase the proliferation rate of damaged SH-SY5Y cells.By contrast, VAPs by micronization method had more effective effect at 62.5μg/mL, and proliferation rate could reach 25.45% .Conclusion Micronization method does not only acquire more protein but also has cell proliferation activity, is a relatively ideal way of protein extraction of velvet antler.
