Study of mechnism and effect of fentanyl on proliferation in gastric cancer cell SGC-7901
- VernacularTitle:芬太尼对胃癌细胞SGC-7901增殖影响及机制研究
- Author:
Haifang ZHANG
;
Xing WEI
;
Haihua CHEN
;
Jia ZHANG
;
Tingjuan SHI
;
Xueling WANG
- Publication Type:Journal Article
- Keywords:
fentanyl;
gastric cancer cell SGC-7901;
proliferation;
NF-κB signal pathway
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(9):38-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore mechnism and effect of fentanyl on proliferation of gastric cancer SGC-7901 cell.Methods Gastric cancer SGC-7901 cell was cultured with fentanyl of 0 (negative control), 0.5, 5 and 50 nmol/L, MTT method was used to detect the effect of fentanyl on SGC-7901 viability.The effect of fentanyl on SGC-7901 cell cycle was measured by flow cytometry.The level of cell related protein,cell cycle protein cyclin D1, Bcl-2.Results Compared with control group, fentanyl (0.5, 5, 50 nmol/L) could inhibit SGC-7901 cell viability, and the inhibitory rate was highest at 48 h.0.5, 5, 50 nmol/L fentanyl made cell cycle arrested in G1 phase.Compared with control group, fentanyl can significantly inhibit cyclinD1 and Bcl-2 expression with drug concentration increasing(P<0.05).Conclusion These results suggeste fentanyl inhibit proliferation of gastric cancer SGC-7901 cell.
