Protective effect of ganlioside GM1 on rats with acute brain trauma and its relevant mechanism
- VernacularTitle:神经节苷脂GM1对大鼠急性脑损伤的保护作用及相关机制研究
- Author:
Bo ZHANG
;
Likun QI
;
Lixin LI
- Publication Type:Journal Article
- Keywords:
monosialoganglioside;
acute brain trauma;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(9):48-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of monosialoganglioside (GM1) on rats with acute brain trauma and its relevant mechanism.Methods Localized brain contusion model in rats were constructed by Feeney's method.65 SD rats were randomly divided into three groups: sham-operation group (n=5), brain injury group (n=30) and GM1 group (n=30).The rats were killed at 3, 7, 14, 28, 56, 168 h after administration, 5 rats in each group (1 rats in sham-operation group).Bax and Bcl-2 protein expression and PARP were decected by immunohistochemical method.The neuronal apotosis was detected by TUNEL.Results There were significant differences in expression of Bax and Bcl-2 protein between brain injury group and sham-operation group at each time point (P<0.05).There were significant differences in expression of Bax and Bcl-2 protein between GM1 group and brain injury group at each time point ( P <0.05 ) , while there were no significant differences in expression of Bax and Bcl-2 protein after 14 h between GM1 group and sham-operation group.After administration, the Bax/Bcl-2 values decreased and was obvious at 14 h.Degradation of PARP and rate of neuronal apotosis in brain injury group at each time point were significantly higher than those in sham-operation group (P<0.05).Degradation of PARP in GM1 group at 28 h, 56 h, 168 h were significant lower than those in brain injury group (P<0.05), and rate of neuronal apotosis was lower at each time point than those in brain injury group (P<0.05).Conclusion GM1 could reduce value of Bax/Bcl-2, degradation of PARP and apoptosis in rats with traumatic injury brain.
