Genotyping of Acinetobacter calcoaceticus-Acinetobacter baumannii complex and analysis of antibiotics resistance
10.3969/j.issn.1673-4130.2015.19.012
- VernacularTitle:醋酸钙-鲍曼不动杆菌复合菌的分子流行病学及体外药物敏感性分析
- Author:
Min LI
;
Wenwen PI
;
Yi WANG
;
Weiping LU
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumanni;
complex group;
gene sequencing;
drug resistance
- From:
International Journal of Laboratory Medicine
2015;(19):2803-2805
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify strains of Acinetobacter(A.)calcoaceticus-A.baumannii complex accurately,and investigate the species distribution of Acinetobacter calcoaceticus-A.baurnannii(ACB)complex isolated in Daping Hospital.Analysis the anti-biotics resistance of ACB complex.Methods A total of 95 clinica1 isolates of ACB complex were collected from Daping Hospital from February to April 2014,identify by sequence analysis of 1 6S rRNA gene spacer region.Use VITEK-2compact automatic iden-tification of bacteria by susceptibility meter to detect the precise identification of all ACB complex group of drug sensitivity.Results Among all the 95 strains of ACB complex identification results were A.baumanni (81,85.26%),A.pittii(10,10.53%),A.noso-comialis (4,4.21%).A.baumannii to third generation cephalosporins,aminoglycosides and carbapenem antibiotic resistance in seri-ous condition,the sensitive rate was only about 20.00%.But A.pittii and A.nosocomialis had a high sensitivity rate to commonly used antibiotics.Conclusion VITEK-2 Compact automatic identification of bacteria susceptibility meter has limitations in identifica-tion of Acinetobacter,with analysis of 1 6S rRNA gene sequencing,the ACB complex could be accurately identified.There is signifi-cant difference in the composite group-resistant characteristics of each species,clinical infection is also various.
