Influence on inflammation cytokines in passive immunotherapy for allergic rhinitis guinea pigs
10.3969/j.issn.1000-484X.2015.10.012
- VernacularTitle:被动免疫治疗过敏性鼻炎豚鼠对炎症细胞因子的影响
- Author:
Xiling ZHU
;
Wenyun ZHOU
;
Hailing ZHANG
;
Jingwu ZHAI
;
Cuiying SUN
;
Qiu CHANG
;
Zhu WEN
;
Guozhu HU
- Publication Type:Journal Article
- Keywords:
Allergic rhinitis;
Guinea pigs;
Ovalbuamin;
TNF-α;
IL-1β;
Cytokines
- From:
Chinese Journal of Immunology
2015;(10):1352-1356
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence on inflammation cytokines for anti-IL-1βand TNF-αIgY intranasal treatment in guinea pigs with allergic rhinitis.Methods:The allergic rhinitis model in guinea pigs was established using ovalbumin(OVA).Hartley guinea pigs were randomly divided into the control group(group C,n=17),the allergic rhinitis model group(group M,n=27),the 0.1%anti-IL-1βand TNF-αIgY treatment group(group Z1,n=21)and the fluticasone propionate treatment group(group Z2,n=21). At 2 h,4 h and 8 h after the last treatment,blood was got by heart puncture,as well as nose was lavaged using 0.9% saline and the nasal lavage fluid( NLF) was collected.The level of cytokines was examined using ELISA kits.Results: In the peripheral blood, the levels of IL-1β,IL-5,IL-9,IL-13,IL-18,IL-33 and TGF-β1 from 2 h to 8 h;TNF-αand OVA-specific IgE from 2 h to 4 h;and IL-22 from 4 h to 8 h were significantly decreased in the 0.1%anti-IL-1βand TNF-αIgY treatment group compared with the allergic rhinitis model group(P<0.05).In the NLF,the levels of IL-1β,IL-5,IL-9,IL-13,IL-22,IL-33,TNF-α,TGF-β1 and OVA-specific IgE from 2 h to 8 h;and IL-18 at 2 h were significantly decreased in the 0.1% anti-IL-1βand TNF-αIgY treatment group compared with the allergic rhinitis model group ( P<0.05 ) .Conclusion: Anti-IL-1βand TNF-αIgY intranasal treatment can significantly reduce inflammation cytokine levels in allergic rhinitis guinea pigs.
