Preparation of Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine
10.3969/j.issn.1000-484X.2015.10.014
- VernacularTitle:3型肺炎球菌荚膜多糖结合疫苗的研制
- Author:
Tao ZHANG
;
Xin MENG
;
Tao ZHU
;
Zheng LIU
;
Liping ZHANG
;
Jieqing HAO
- Publication Type:Journal Article
- Keywords:
Streptococcus pneumonia type 3 capsular polysaccharide;
Conjugate vaccine;
Aluminum adjuvant;
Immunogenicity
- From:
Chinese Journal of Immunology
2015;(10):1361-1365
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine.Methods:Strep-tococcus pneumonia type 3 capsular polysaccharide was covalently linked to protein CRM197 and its immunogenicity was evaluated in infant mice.Results:Through the preliminary research, we found that the polysaccharide was treated with 0.2 mol/L acetic acid at 85℃ for 1 h,activation grade reached to 10.0,and the radio of polysaccharide and protein was 20∶10,could induce infant mice to produce the high titers of antibodies.Conclusion:This result shows that conjugate vaccine prepared under this condition retained intact antigenicity.It is applicable to prepare Streptococcus pneumonia type 3 capsular polysaccharide conjugate vaccine by the process condi-tions.