Analyze curative effect of suppression expression of URG4 on cervical cancer mice and possible mechanisms
10.3969/j.issn.1000-484X.2015.10.023
- VernacularTitle:抑制URG4表达对宫颈癌小鼠的治疗作用及机制
- Author:
Xi ZHOU
;
Zhongdong CHEN
;
Lu HE
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
Upregulated gene 4;
Wnt/β-catenin signaling pathway
- From:
Chinese Journal of Immunology
2015;(10):1400-1403
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the curative effect of suppression expression of URG4 on cervical cancer mice and the possible mechanisms.Methods:The expression of URG4 were analyzed in cervical cancer tissues and para-carcinoma tissues.16 male BALB/c nude mice were divided into Group A ( inoculated with URG4-siRNA SiHa cells) and Group B ( inoculated with SiHa cells) . After 4 weeks,mice were executed and analyzed.Results:The expression of URG4 were higher in human cervical cancer tissues than that in para-carcinoma tissues ( P<0.05).After experiment,Group A had a lower tumor weight,but a higher body weight than these of Group B,the difference was statistically significant (P<0.05).Group A mice had a lower levels of URG4 in tumor than that of Group B mice (P<0.05).Group A mice had a lower levels of Ki67 in tumor than that of Group B mice,the difference was statistically significant (P<0.05).Group A mice had lower levels of CyclinD1,β-catenin and C-myc in tumor than these of Group B mice (P<0.05). Conclusion:The expression of URG4 in cervical cancer tissue is up-regulation.Suppression expression of URG4 can suppress tumor growth in vivo,which may though suppress the Wnt/β-catenin signaling pathway.
