Effects of EPO on expression of TGF-β1 and ColⅠin myocardium of liver cirrhosis rats
10.3969/j.issn.1001-1978.2015.10.015
- VernacularTitle:促红细胞生成素对肝硬化大鼠心肌TGF-β1及ColⅠ基因表达的影响
- Author:
Weiping ZHANG
;
Ying YU
;
Qin GAO
;
Xiaolei GUO
;
Sudong GUAN
- Publication Type:Journal Article
- Keywords:
liver cirrhosis;
myocardium;
EPO;
colla-gen;
cardiac function;
TGF-β1
- From:
Chinese Pharmacological Bulletin
2015;(10):1398-1402
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effects of liver cirrho-sis on the expression of transforming growth factor-β1 ( TGF-β1 ) and ColⅠin rat myocardium and interven-tion of erythropoietin ( EPO ) . Methods Thirty-six male Sprague-Dasley rats were randomly divided into three groups:control group, liver cirrhosis group and EPO group, then the cardic hemodynamic parameters in vivo and levels of serum lactate dehydrogenase ( LDH ) as well as creatine kinase isoenzyme ( CK-MB) were measured. With Masson′s trichrome stain, changes of collagen formation of myocardial tissue in different groups were observed. Also the mRNA ex-pressions of TGF-β1 and ColⅠin myocardium were de-tected by RT-PCR. Results In contrast to control group, rats in liver cirrhosis group showed a decline in systolic and diastolic function of left ventricule, rising myocardial enzyme, a distinct increase of cardiac colla-gen deposition, as well as an elevation of TGF-β1 and ColⅠmRNA expressions. In contrast to liver cirrhosis group, rats in EPO group demonstrated an improve-ment in systolic and diastolic function of left ventricule as well as in cardiac collagen deposition, and a de-crease in both myocardial enzyme and TGF-β1 and ColⅠmRNA expressions. Conclusion Liver cirrhosis can lead to the changes of myocardial structure and function in rats,and it can accelerate myocardial inter-stitial fibrosis; EPO can protect the myocardial injury in liver cirrhosis rats.
