Isoliquiritigenin induced apoptosis in human melanoma A375 cells
10.3969/j.issn.1001-1978.2015.10.020
- VernacularTitle:异甘草素诱导人黑色素瘤A375细胞凋亡研究
- Author:
Xinyan YAN
;
Lingling SI
;
Caixia GAO
;
Lina YU
;
Yanming WANG
;
Qiusheng ZHENG
- Publication Type:Journal Article
- Keywords:
isoliquiritigenin;
human melanoma cells;
cell apoptosis;
apoptotic morphology ROS;
apoptotic morphology;
mitochondrial membrane potential;
gly-colysis
- From:
Chinese Pharmacological Bulletin
2015;(10):1426-1432
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the mechanism of apopto-sis induced by the isoliquiritigenin in A375 human ma-lignant melanoma cells. Methods Sulforhodamine B ( SRB) method was used to determine the A375 cell viability;acridine orange/ethidium bromide ( AO/EB) and Hoechst 33258 staining were used to observe the morphological changes of apoptotic cells; flow cytome-try was used to detect A375 cell apoptotic rate;DCFH-DA was applied to determine the changes of total intra-cellular ROS in A375 cells;JC-1 method was used to measure the changes of mitochondrial membrane poten-tial;the kits methods were used to determine the con-tent of ATP, lactic acid and glucose in A375 cell which was treated with different concentrations of isoliquiritigenin. Results Isoliquiritigenin could in-hibit A375 cell proliferation in a concentration-depend-ent manner; A375 cells showed obvious apoptosis charateristics after treatment by isoliquiritigenin, and the apoptosis rate increased with increasing concentra-tion of isoliquiritigenin. The level of total intracellular ROS in A375 cells increased obviously after dealing with different concentrations of isoliquiritigenin;in ad-dition, the mitochondrial membrane potential, the lev-els of intracellular ATP,lactic acid and the level of glu-cose uptake all declined. Conclusions These find-ings demonstrate that isoliquiritigenin can induce apop-tosis of A375 cells. The mechanism may be related to elevation of ROS level and reduction of aerobic glycoly-sis level.
