Pharmacokinetic study on cimifugin in rats
10.3969/j.issn.1001-1978.2015.10.023
- VernacularTitle:升麻素在大鼠体内的药代动力学研究
- Author:
Xiaoyu WANG
;
Xiaoyan JIANG
;
Xi YU
;
Hailiang LIU
;
Yan WANG
;
Yu TAO
;
Min HONG
- Publication Type:Journal Article
- Keywords:
cimifugin;
HPLC;
rat;
pharmacokinet-ics;
methodology;
allergic dermatitis
- From:
Chinese Pharmacological Bulletin
2015;(10):1443-1446
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a HPLC method for de-termining cimifugin in rat plasma and investigate the pharmacokinetic characteristics of cimifugin in rats. Methods The plasma concentration of cimifugin was detected by HPLC in acetonitrile protein precipitation method after intragastric administration of cimifugin. The pharmacokinetic parameters were calculated by the procedure of DAS 2 . 1 . Results The regression equa-tion of cimifugin in rats plasma was Y =0. 187 X -0. 0236 (R2 =0. 998 2),which shows a good linear re-lation at 1 - 70 mg · L-1 . The concentration-time curves conformed to two-compartment model. The main pharmacokinetic parameters of Tmax, Cmax, T1/2α, T1/2z, Vd ,AUC(0-t) and AUC(0-∞) were 80 min, 10. 359 mg ·L-1 , 93. 131 min, 2. 179 L · kg-1 , 1946. 085 mg ·L-1 · min, 2138. 57 mg · L-1 · min, respectively. Conclusions We established a HPLC method to de-termine the concentration of cimifugin in plasma. The method is so highly specified and sensitive that it can be used in quantitative analysis in vivo on cimifugin. Cimifugin can be rapidly absorbed, reach the highest concentration and produce effect.
