ALEX1 expression in cervical cancer tissues and effect of ALEX1 on cervical cancer cell biology behavior
10.3969/j.issn.1001-1978.2015.10.024
- VernacularTitle:ALEX1在宫颈癌中的表达及对宫颈癌细胞生物学行为的影响
- Author:
Fan ZENG
;
Jiayan WU
;
Yue GAO
;
Hantao ZHANG
;
Xin BAI
;
Geli LIU
;
Fangzhou SONG
- Publication Type:Journal Article
- Keywords:
ALEX1;
cervical cancer;
resveratrol;
siRNA;
cell proliferation;
cell apoptosis;
cell cycle
- From:
Chinese Pharmacological Bulletin
2015;(10):1447-1451
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate ALEX1 gene expres-sion in cervical cancer tissues and adjacent non-can-cerous tissues, and to explore the ALEX1 genetic influ-ence on cell proliferation,cycle and apoptosis of human cervical cancer cell line HeLa. Methods ALEX1 protein expression in cervical cancers and in non-can-cerous cervical tissues was evaluated using immunohis-tochemical method. A small interference RNA targeting ALEX1 gene was transfected into HeLa cells′, and the effect of ALEX1 interference on HeLa cells′ cycle and apoptosis was analysed by flow cytometry. The effect of ALEX1 interference on HeLa cells′ proliferation and sensitivity to resveratrol was analysed by CCK-8 assay. Results ALEX1 protein expression was significantly increased in cervical cancer tissues compared with non-cancerous tissues. HeLa cells′proliferation was inhibi-ted compared with control group and blank group. He-La cells′ sensitivity to resveratrol was enhanced com-pared with control group blank group. Conclution SiRNA silencing of ALEX1 gene could significantly in-hibit HeLa cells′ proliferation and enhance resveratrol ability of inhibiting HeLa cells′proliferation.
