Simultaneous Determination of Imperatorin, Isoimperatorin and Magnolin in Quchuan Capsules by HPLC
- VernacularTitle:HPLC法同时测定祛喘胶囊中木兰脂素、欧前胡素、异欧前胡素的含量
- Author:
Wang JING
- Publication Type:Journal Article
- Keywords:
HPLC;
Quchuan capsules;
Imperatorin;
Isoimperatorin;
Magnolin
- From:
China Pharmacist
2015;18(10):1832-1834
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method to simultaneously determine the content of imperatorin, isoimperatorin and magnolin in Quchuan capsules by HPLC. Methods:A Shiseido CAPCELL PAK C18 column(150 mm × 4. 6 mm,5 μm) was used. The mobile phase consisted of acetonitrile(A)-water(B) with gradient elution at a flow rate of 1. 0 ml·min-1 and the column temperature was at 30℃. The detection wavelength was set at 278 nm from 0 to 25 min and 248 nm from 25 to 70 min. The injection volume was 10 μl. Results:The linear range of imperatorin,isoimperatorin and magnolin was 6. 126-55. 134 μg·ml-1(r=0. 999 7), 9. 862-88. 758 μg ·ml-1(r =0.999 8) and 4.830-43.470 μg·ml-1(r =0.999 8), respectively. The average recovery was 101.5% (RSD =1. 4%), 99. 6%(RSD=1. 2%) and 100. 2% (RSD=1. 7%), respectively. Conclusion: The method is convenient, sensitive and accurate, which can be used for the quality control of Quchuan capsules.
