Simultaneous Determination of Gallic Acid and Piperine in Mongolian Medicine Naru Sanwei Pills by HPLC
- VernacularTitle:HPLC法测定蒙药那如三味丸中没食子酸和胡椒碱的含量
- Author:
Li LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Naru Sanwei pills;
Gallic acid;
Piperine
- From:
China Pharmacist
2015;18(10):1830-1832
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a simultaneous determination method for gallic acid and piperine in Naru Sanwei pills. Meth-ods:The separation was carried out on an Ultimate XB-C18 (250 mm × 4. 6 mm,5 μm) column. The mobile phase was methanol and 0. 1% phosphoric acid with gradient elution. The gradient program was as follows: in 0-20 min, methanol was changed from 15% to 80%;in 20-30 min, methanol was changed from 80% to 15%. The flow rate was 1. 0 ml·min-1 . The detection wavelength was 285 nm, the column temperature was 30℃,and the sample size was 10μl. Results:The linear range of gallic acid and piperine was 0. 878-4. 392 μg(r=1. 000 0)and 0. 262-1. 308 μg(r=1. 000 0), respectively. The average recovery was 100. 75%(RSD=1. 19%) and 98. 35%(RSD=1. 46%)(n=9), respectively. Conclusion:The method with high precision and resolution can be used for the quali-ty control of Naru Sanwei pills.
