Production Of Gm-Csf And Tgf-beta 1 In Irradiated Human Gingival Fibroblasts Cultured With Lipopolysaccharide.
- Author:
Hong Sik KIM
1
;
Seong Geun LEE
;
Kwang Hyuk KIM
;
Uk Kyu KIM
;
Jong Ryoul KIM
;
In Kyo CHUNG
;
Dong Kyu YANG
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University, Korea. omslee@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Gingival fibroblasts;
Irradiation;
lipopolysaccharide
- MeSH:
Enzyme-Linked Immunosorbent Assay;
Fibroblasts*;
Fibrosis;
Granulocyte-Macrophage Colony-Stimulating Factor*;
Humans*;
Mouth Neoplasms;
Mucositis;
Transforming Growth Factor beta*;
Transforming Growth Factor beta1
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2002;28(3):169-174
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Irradiation in the oral cancer patients causes early and late complications such as intraoral mucositis and fibrosis, with a various expression of GM-CSF and TGF-beta. The purpose of this study was to investigate the production of GM-CSF and TGF-beta1 by the irradiated human gingival fibroblasts cultivated with lipopolysaccharide. MATERIALS AND METHODS: Irradiated (total dose, 60 Gy) human gingival fibroblasts were incubated with LPS. Culture supernatants that were collected at 24, 48, and 72 hours were assessed for GM-CSF and TGF-beta1 by enzyme-linked immunosorbent assay. RESULTS: 1. GM-CSF production in nomal gingival fibroblasts was increased with incubation time, but decreased with incubation time in irradiated gingival fibroblasts. GM-CSF production in both normal and irradiated gingival fibroblasts induced with LPS was higher than the control. 2. TGF-beta1 production in normal gingival fibroblasts was decreased after 24 hours, but, it was increased until 48 hours in irradiated gingival fibroblasts. TGF-beta1 production in normal gingival fibroblasts exposed with LPS was higher than the control. Conversely, It was lower than the control in irradiated gingival fibroblasts exposed with LPS. CONCLUSION: This indicates that irradiation in gingival fibroblasts may play an important role in radiation-induced intraoral mucositis and fibrosis. However, LPS decreases the production of TGF-beta1 in the irradiated gingival fibroblasts.
