Experimental study of in vitro construction of engineering myocardial tissue based on rat BMSCs induced by Ang II and accellular bovine pericardium
10.3760/cma.j.issn.1673-4181.2015.03.002
- VernacularTitle:AngⅡ诱导大鼠BMSCs联合脱细胞牛心包体外构建工程化心肌组织的实验研究
- Author:
Jingbao SHI
;
Wanhong XING
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Cardiomyocyte;
Tissue engineering;
Angiotensin II
- From:
International Journal of Biomedical Engineering
2015;(3):133-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of inducing rat bone marrow mesenchymal stem cells ( BMSCs ) into myocardial cells by angiotensin II ( Ang II ) and the approach of applying the induced BMSCs to construct myocardial tissue in vitro. Methods BMSCs from rats were isolated and cultured. The third generation of BMSCs were incubated in complete culture medium, in which 0.1 μmol/L Ang II was added, for 24 h. The BMSCs were switched to complete medium and cultured. The control group was cultured by complete medium without Ang II . The protein cTnT and β-MHC were tested by immunofluorescence staining and the structure of induced cells was observed by transmission electron microscopy. The bovine pericardiums were managed by the detergent-enzyme digestion method. The induced BMSCs were planted onto the acellular bovine pericardium and cultured for 3 d before being tested. Results The cells of induced group were induced into cardiomyocytes by Ang II, which could express cTnT andβ-MHC, while cells in the control group couldn't. The result of electron microscopy showed that desmosome could be observed in the induction group. The surface of acellular bovine pericardiums was detected by scanning electron microscopy and no cells remaining on the surface except 2 μm-aperture were observed in the acellular bovine pericardiums. The induced BMSCs adhered to acellular bovine pericardium and grew well. Conclusions BMSCs can be induced into cardiomyocyte in vitro and express cTnT and β-MHC. The cells can adhere well on the surface and the inside of acellular bovine pericardium. BMSCs have the potential to form engineered cardiac muscle tissue with blood vessel.
