Effects of adenovir us mediated AdipoQ on mice submandibular gland in Sj?gren's syndrome
10.3760/cma.j.issn.1007-7480.2015.07.008
- VernacularTitle:腺病毒介导脂联素对干燥综合征小鼠颌下腺炎症的影响
- Author:
Meng CHEN
;
Ke XU
;
Liyun ZHANG
;
Gailian ZHANG
;
Dan MA
;
Jianwen HOU
;
Jiangong LIU
;
Zhenhua ZHANG
- Publication Type:Journal Article
- Keywords:
Sj?gren's syndrome;
Submandibular gland;
Adenovirus vector
- From:
Chinese Journal of Rheumatology
2015;(7):464-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of AdipoQ in Sj?gren's syndrome (SS) mice and its role in inflammation was investigated by recombinant gene transfection study in vivo. Methods As spon-taneous SS mice model, a total of 30 NOD mice were divided into 3 groups randomly: recombinant adenovirus (rADV-AdipoQ) group, normal saline control and simple adenoviruses (control group). The submandibular gland morphology, histopathological grading and the level of serum tumor necrosis factor-α (TNF-α), AdipoQ was compared between the three groups. The expression of AdipoQ on mice submandibular gland was assessed by means of semi-quantitative reverse transcriptase polymerase chain reaction. Results The submandibular glands of the mice of the control group were destructed, with focal lymphocytic infiltration and acinus atrophy. Compared with control model groups, the serum TNF-α and salivary gland AdipoQ expression was significantly down-regulated in the rADV-AdipoQ group [(248 ±30) vs (162 ±73) ng/ml] (P<0.05). Conclusion AdipoQ gene transfected SS mice can significantly improve the morphological features of tissues and decrease the concentration of TNF-αin serum, in addition, it can effectively inhibit inflammation, decrease the degree of protein and AdipoQgene expression. So the AdipoQ may be the protective gene in SS mice.
