Differentiation of human placenta mesenchymal stem cells into insulin-producing cells
10.3760/cma.j.issn.1673-4181.2015.03.007
- VernacularTitle:人胎盘间充质干细胞诱导分化为胰岛素分泌细胞的研究
- Author:
Yan LU
;
Juan WANG
;
Yuan ZHANG
- Publication Type:Journal Article
- Keywords:
Placenta;
Mesenchymal stem cells;
Differentiation;
Islet;
Diabetes
- From:
International Journal of Biomedical Engineering
2015;(3):156-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the methods of inducing human placenta mesenchymal stem cells (MSCs) to differentiate into insulin-producing cells. Methods MSCs were obtained from human placenta and were induced to differentiate into insulin-producing cells by activin A, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and other cytokines. The cells were identified by immunocytochemistry staining, Western Blot and animal experiment after differentiation. Results After induction, the cells possessed some characteristics of islet βcells, and could express pancreatic duodenal homeobox-1 (PDX-1), insulin and C peptide. The induced cells could also decrease blood glucose levels of diabetic mice. Conclusions These results showed that human placenta MSCs can be induced to differentiate into insulin-producing cells by cytokines.
