Usefulness of PCR-SSP Method for Detection of HLA-DRB1*0405.
- Author:
Young Hoon KIM
1
;
Kyung Hee LEE
;
Oh Joong KWON
;
Jeong Ho SEOK
;
Young Suk CHO
Author Information
1. SamKwang Medical Laboratories Research & Development Team, Seoul, Korea. yscho@smlab.co.kr
- Publication Type:Original Article
- Keywords:
HLA-DR;
PCR-SSP;
Rheumatoid arthritis;
HLA-DRB1*0405
- MeSH:
Alleles;
Arthritis, Rheumatoid;
HLA-DR Antigens;
HLA-DRB1 Chains;
Humans
- From:Journal of Laboratory Medicine and Quality Assurance
2007;29(1):201-205
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The association between HLA-DRB1 gene and severity of rheumatoid arthritis (RA) has been documented in various reports. Especially, DRB1*0405 allele shows significant association with RA in Korean patients. DRB1*0405 typing has been performed by the sequence based typing method (SBT), that is a difficult and expensive method. So we tried to replace it with a simple and inexpensive method. METHODS: We performed the HLA-DRB1*0405 typing using PCR-SSP technique with sequence specific primers of 3 pair in 298 Koreans. These results were compared with those of high resolution sequence based typing (SBT) method. RESULTS: Of 298 samples typed with the high resolution SBT method, 60 samples were HLA-DRB1*04 positive and showed 9 subgroups of HLA-DRB1*04 and 26 samples were HLA- DRB1*0405 positive. With the PCR-SSP method, same 26 samples were HLA-DRB1*0405 positive, showing 100% correspondence between two methods to detect HLA-DRB1*0405 CONCLUSIONS: Using PCR-SSP method to type HLA-DRB1*0405 is a very useful tools for studying the association between rheumatoid arthritis and HLA-DRB1*0405 from a practical and economic view.
