Effect of Liraglutide on Toll-like Receptor 4 and Na+/K+-ATPase Activity in Proximal Tubular Epithelial Cells of Rats Induced by High Glucose
10.3870/yydb.2015.08.003
- VernacularTitle:利拉鲁肽对高糖诱导大鼠近端小管上皮细胞Toll 样受体4表达和钠钾ATP酶活性影响
- Author:
Xinghong WANG
;
Yaping ZHENG
;
Lifei WANG
- Publication Type:Journal Article
- Keywords:
Liraglutide;
Proximal tubule epithelial cells;
Na+/ K+-ATPase;
Inflammation;
Toll-like receptor 4
- From:
Herald of Medicine
2015;(8):998-1001
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of liraglutide ( Li) on the proximal tubular cells ( PTECs) of rats induced by model control. Methods The PTECs of rats were obtained by primary culture and divided into normal control group ( NC) , model control group ( HG) , HG+low-dose Li group, HG+medium-dose Li group, and HG+high-dose Li group ( n-6 in each group) . After 72 h of incubation, the activity of Na+/K+-ATPase in PTECs was measured by the liquid scintillation counter. Protein expression of Toll-like receptor 4 ( TLR4 ) was measured by Western blotting. Interleukin-6 ( IL-6 ) , tumor necrosis factor-α (TNF-α) and plasminogen activator inhibitor-1 (PAI-1) in culture supernatants were measured by the enzyme linked immunosorbent assay (ELISA). Results Compared with NC, activity of Na+/K+-ATPase [(2 737. 88±317. 22)μmol·g-1 ·h-1 ] and the protein expression of TLR4 in PTECs, IL-6, TNF-α and PAI-1 in culture supernatants were significantly increased in HG (P<0. 05, or P<0. 01). Compared with HG, activity of Na+/ K+-ATPase and the protein expression of TLR4 in cells, IL-6, TNF-αand PAI-1 in culture supernatants reduced significantly in HG+medium-or high dose Li groups (P<0. 05, or P<0. 01). Conclusion Liraglutide can inhibit the expression of inflammatory cytokines and stabilize the Na+/ K+-ATPase’ s activity in PTECs in high glucose environment. Therefore, it may play a role in kidney protection.
