Effect of Bcl-2 knockdown on apoptosis and drug sensitivity of 5-FU in thegastric carcinoma HGC-27cells
10.3969/j.issn.1006-5725.2015.15.004
- VernacularTitle:剔除Bcl-2基因对胃癌细胞HGC-27凋亡及5-氟尿嘧啶敏感性的影响
- Author:
Xiaoming OUYANG
;
Kunhe WU
;
Tao ZENG
- Publication Type:Journal Article
- Keywords:
Gastric Carcinoma;
Bcl-2;
siRNA;
5-FU;
apoptosis
- From:
The Journal of Practical Medicine
2015;31(15):2423-2426
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Bcl-2 gene knockdown on apotosis , proliferation and drug sensitivity of gastric carcinoma HGC-27 cells. Methods The HGC-27 cells were divided into five groups:the untreated control group , the control siRNA group , the specific siRNA targeting Bcl-2 gene group , 5-FU treated group and the combination group (Bcl-2 siRNA and 5-FU treatment). Then flow cytometry and MTT assays were performed to detect the apoptosis and proliferation of HGC-27 cells. The cysteine protease activityand Cytochrome C release level were tested by ELISA method. Results Bcl-2 knockdown enhanced apoptosis and inhibited proliferation of HGC-27 cells. Comparedwith the 5-FU-treated group , the cell apoptosis level, activities of Caspase-3 and Caspase-9, plasma Cytochrome C were significantly increased in the combination group(P <0.01). Conclusion Bcl-2 gene knockdown induced apoptosis, inhibited cell proliferation and enhanced the drug sensitivity of 5-FU in gastric cancer cells , which might be considered as a potential therapeutic strategy forgastric carcinoma.
