Effect of mesenteric lymphatic ligation on liver injury induced by severe acute pancreatitis in rats
10.3969/j.issn.1006-5725.2015.14.004
- VernacularTitle:肠系膜淋巴管结扎对重症急性胰腺炎大鼠肝损伤的影响
- Author:
Yuanqi LIU
;
Lulu WANG
;
Li CHEN
;
Xiaoyong TAN
;
Ling FAN
;
Yuxia XIONG
- Publication Type:Journal Article
- Keywords:
Mesenteric lymph duct;
Ligation;
Severe acute pancreatitis;
Liver injury
- From:
The Journal of Practical Medicine
2015;31(14):2252-2254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of mesenteric lymphatic ligation on liver injury induced by severe acute pancreatitis. Methods 54 SD rats are randomly divided into sham-operation group (A), severe acute pancreatitis group (B) and severe acute pancreatitis and ligation group (C). Liver tissues were collected from each rat in 6, 12, and 24 h after ligation. Pathological changes in liver tissues were observed by haematoxylin and eosin staining. Levels of serum glutamic-pyruvic transaminase and glutamic oxalacetic transaminase were detected. Levels of TNF-α and IL-1 in liver homogenate were examined by enzyme linked immunosorbent assay. Results Under light microscopic examination, neutrophils and inflammatory exudate in hepatic lobule was increased as time prolonged in group B. Inflammation was reduced in group C as compared with group B.Levels of serum glutamic-pyruvic transaminase and glutamic oxalacetic transaminase were more significantly increased in groups B and C than in group A (P<0.05);while they were more significantly decreased in group C than in group B. Levels of TNF-αand IL-1 in liver homogenate were significantly increased in group B as compared with group A (P < 0.05); whereas they were more significantly decreased in group C than in group B at 24 h (P < 0.05). Conclusions Mesenteric lymphatic ligation can reduce liver injury in severe acute pancreatitis. Mesenteric lymphatic fluids may play an important role in severe acute pancreatitis.
