Effects of 1,25-Dihydroxyvitamin D3 on Alveolar Fluid Clearance in Mice
- VernacularTitle:1,25-二羟基维生素D3在小鼠肺脏液体清除中的作用
- Author:
Xin GE
;
Yong CUI
;
Yan DING
;
Bing LIU
;
Sihui WU
;
Hongguang NIE
- Publication Type:Journal Article
- Keywords:
vitamin D;
pulmonary edema;
alveolar fluid clearance;
epithelial sodium channel
- From:
Journal of China Medical University
2015;44(8):679-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between 1,25-dihydroxyvitamin D3(1,25-VD3)and alveolar fluid clearance(AFC)in mice in vivo,and investigate its effects in the process of lung fluid clearance. Methods KM male mice were treated with active vitamin D analogue parical-citol(daily i.p. injection)for 2 weeks,and then the in vivo AFC of these mice was measured by bovine serum albumin protein assays. western blot was applied to determine epithelial sodium channel protein levels in lungs of these mice. Results In vivo total AFC was 31.9%±3.8%in vitamin D-treated mice,and significantly lower in the vehicle-treated controls(19.7%±1.9%,P<0.05). Amiloride-sensitive AFC was increased approximate-ly 50%by vitamin D. western blot showed that the expression ofα-epithelial sodium channel was significantly elevated in paricalcitol-treated mouse lungs. Conclusion These observations suggest that vitamin D augments AFC in mice,which may be related to the augment of epithelial sodium channel protein expression. The clinical application of vitamin D therapy may ameliorate pulmonary edema of patients.
