Protective Effect of Intravenous Infusion of Low Dose Minocycline Postconditioning on Myocardial Ischemia-reperfusion Injury in Rat
- VernacularTitle:低剂量静脉注射米诺环素后处理对大鼠缺血再灌注心肌的保护作用
- Author:
Liqun ZHANG
;
Yongjun CHEN
;
Renshan CUI
;
Guoxian QI
- Publication Type:Journal Article
- Keywords:
minocycline;
ischemia postconditioning;
myocardial ischemia-reperfusion injury
- From:
Journal of China Medical University
2015;44(8):685-689
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of low dose intravenous minocycline postconditioning on myocardial ischemia-reperfusion injury in rat,and to investigate the possible mechanisms. Methods Forty-eight male wistar rats were randomly divided into four groups:sham-operation (SO)group,ischemia-reperfusion(IR)group,low-dose minocycline(3 mg/kg,LM)group and high-dose minocycline(10 mg/kg,HM)group. The rat model of myocardial IR was established by occlusion of the left anterior descending coronary artery for 45 minutes and reperfusion for 120 minutes.After the reperfusion,the parameters of haemodynamics were recorded;creatine kinase MB(CK-MB),cardiac troponin-I(cTn-I),malond-ialdehyde(MDA),superoxide dismutase(SOD)in serum and myocardium,myocardial apoptosis index(AI)and the myocardial tissue morphology were determined. Results Compared with IR group,LM and HM treatment significantly reduced the levels of CK-MB form,cTn-I,AI and MDA, lowered LVEDP,enhanced LVSP and ±dp/dtmax,elevated the activity of SOD in serum and myocardium(P<0.05). The effect of HM is stronger than LM on these above mentioned indicators,but the difference was not statistical significance(P>0.05). Conclusion LM postconditioning can protect against myocardial IR injury,and the protective effect may be related to the scavenging of oxide free radical,which further restrain the reac-tion of lipid peroxidation and apoptosis.
