Role of AMP-activated protein kinase in regulating LPS-induced secretion of inflammatory cytokines in mouse alveolar macrophages
10.3760/cma.j.issn.0254-5101.2015.07.005
- VernacularTitle:小鼠肺泡巨噬细胞中腺苷酸活化蛋白激酶参与内毒素诱导炎症因子分泌的调控
- Author:
Wei WANG
;
Xiaoguang LI
;
Jie XU
- Publication Type:Journal Article
- Keywords:
Mice alveolar macrophage;
Adenosine monophosphate-activated protein kinase;
LPS;
Inflammatory cytokine
- From:
Chinese Journal of Microbiology and Immunology
2015;35(7):502-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of 5′adenosine monophosphate-activated pro-tein kinase ( AMPK) in the regulation of LPS-induced secretion of inflammatory cytokines in mouse alveolar macrophages.Methods Alveolar macrophages were isolated from wild type and AMPKα1-/-C57BL/6J mice.ELISA was used to measure the concentrations of IL-1βand TNF-αin the culture supernatants of LPS treated alveolar macrophages with or without 5-amino-1-β-D-ribofuranosyl-imidazole-4-carboxamide ( AICAR ) stimulation.Western blot assay was performed to analyze the expression of AMPKα1 and AMPKα2 in alveolar macrophages isolated from wild type and AMPKα1-/-mice as well as the LPS-induced changes of p-AMPKαactivity in wild type mice.Results The LPS-induced secretion of inflammatory cyto-kines in alveolar macrophages isolated from AMPKα1-/-mice were significantly higher than that of wild type mice.The activities of p-AMPKαin wild type mice were suppressed by LPS treatment.Treatment of AICAR inhibited the LPS-induced secretion of TNF-αand IL-1β.Conclusion The LPS-induced secretion of in-flammatory cytokines was enhanced in AMPKα1-deficient mice, but could be suppressed upon the treatment of AICAR, an agonist of AMPK.AMPK was involved in the regulation of LPS-induced secretion of inflamma-tory cytokines by alveolar macrophages in mice.
