Vascular endothelial growth factor 165 transfection promotes proliferation of adipose-derived mesenchymal stem cells
10.3969/j.issn.2095-4344.2015.36.008
- VernacularTitle:血管内皮生长因子165转染可促进脂肪间充质干细胞增殖
- Author:
Guang SAN
;
Jia SONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(36):5782-5788
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Vascular endothelial growth factor is the most important pro-angiogenic factor, which can promote revascularization and survival of fat grafts during fat transplantation. OBJECTIVE:To investigate the effect of vascular endothelial growth factor 165 transfection on the proliferation of adipose-derived mesenchymal stem cel s. METHODS:Recombinant vascular endothelial growth factor 165 mRNA fragment was transmitted into adenovirus pAdEasy-1 systems that were packaged to measure viral titer (experimental group). Empty adenovirus was also packaged as control group. Two kinds of packaged adenovirus solution were transferred into adipose-derived mesenchymal stem cel s at the best multiplicity of infection=100. Cel s with no transfection served as blank group. RT-PCR and western blot methods were used to detect the expression of vascular endothelial growth factor 165 at mRNA and protein levels;MTT method was adopted to detect cel proliferation. RESULTS AND CONCLUSION:The expressions of vascular endothelial growth factor 165 mRNA and protein were significantly higher in the experimental group than the control and blank groups (P<0.05). The division and proliferation of transfected adipose-derived mesenchymal stem cel s were increased significantly in the experimental group, which was significantly different from the control and blank groups (P<0.05). These finding indicate that vascular endothelial growth factor 165 transfection cannot only sustain the target protein expression of adipose-derived mesenchymal stem cel s, but also promote the proliferation of adipose-derived mesenchymal stem cel s remarkably.
